Characterization of Coat Protein Gene of Cucumber Mosaic Virus Isolates in Iran

Authors

  • Nazanin Arafati Department of Agronomy and Plant Breeding, Sabzevar Branch Islamic Azad University, Sabzevar, IR Iran
  • Reza Pourrahim Department of Plant Virus Research, Iranian Research Institute of Plant Protection, Tehran, IR Iran
  • Shirin Farzadfar Department of Plant Virus Research, Iranian Research Institute of Plant Protection, Tehran, IR Iran
Abstract:

Background: Cucumber mosaic virus (CMV) from the Bromoviridae family, is one of the most widespread plant viruses in the world. Objectives: In the present study tomato fields in Guilan, Isfahan, Khorasan Razavi, Khuzestan and Tehran provinces were surveyed to determine the presence of CMV subgroups during 2011-2012. Materials and Methods: Out of 305 symptomatic leaf samples tested by Enzyme-linked immuno sorbent assay (ELISA), 147 samples (48.2 %) were found to be infected by CMV with the highest percentage in Khorasan Razavi (67.4%) followed by Khuzestan (50.6%), Tehran (48%), Isfahan (38.2%) and Guilan (34.3%). The coat protein (CP) gene in the 19 sequenced CMV isolates composed of 657 nucleotides (nt) in a size that encodes 218 amino acids. Phylogenetic analysis based on the nt CP gene showed that the ToKz1, ToKz2, ToKz3 and ToKz4 from Khuzestan fell into subgroup IB and the rest of the Iranian isolates including those sequenced in this study fell into subgroup IA. Results: Subsequent analyses showed that the Iranian CMV isolates belonging to subgroup IA of CMV were most related phylogenetically to each other and they were distinct from the subgroup IB and subgroup II isolates. Bioassay on Nicotiana glutinosa and Solanum lycopersicum showed that the symptoms caused by subgroup IB isolates from Khuzestan were milder than those caused by CMV isolates from subgroup IA under this study. Conclusions: In Iran only subgroups IA and II have been reported, however for the first time this study shows the occurrence and phylogenetic relationships of CMV subgroup IB isolated from tomato fields in West Asia, Iran.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

characterization of coat protein gene of cucumber mosaic virus isolates in iran

background: cucumber mosaic virus (cmv) from the bromoviridae family, is one of the most widespread plant viruses in the world. objectives: in the present study tomato fields in guilan, isfahan, khorasan razavi, khuzestan and tehran provinces were surveyed to determine the presence of cmv subgroups during 2011-2012. materials and methods: out of 305 symptomatic leaf samples tested by enzyme-lin...

full text

Characterization of Cucumber Mosaic Virus From Mint in Tehran Province

Cucumber mosaic virus (CMV) is one of the causal agents of viral diseases that infected mint (Mentha spp.). CMV is a member of the cucumovirus genus in  Bromoviridae family. The virus is naturally transmitted by aphids in a non-persistent manner.During  growing season 2007-2008, leaf samples from plants of mint fields with symptoms of viral disease were collected in mint fieldas in Tehran provi...

full text

Characterization of Cucumber Mosaic Virus From Mint in Tehran Province

Cucumber mosaic virus (CMV) is one of the causal agents of viral diseases that infected mint (Mentha spp.). CMV is a member of the cucumovirus genus in  Bromoviridae family. The virus is naturally transmitted by aphids in a non-persistent manner.During  growing season 2007-2008, leaf samples from plants of mint fields with symptoms of viral disease were collected in mint fieldas in Tehran provi...

full text

Characterization of the Full Length Coat Protein Gene of Iranian Grapevine fanleaf virus isolates, genetic variation and phylogenetic analysis

The full-length coat protein gene of Grapevine fanleaf virus (GFLV) isolates from Iran was characterized byreverse transcription polymerase chain reaction (RTPCR) and sequencing. The expected 1515 bp coatprotein (CP) gene amplicon was obtained for 16 isolates out of 89 that were identified by double antibodysandwich enzyme-linked immunesorbent assay (DASELISA) in a population ...

full text

Phylogenetic analysis of two Iranian grapevine virus A isolates using coat protein gene sequence

Symptomatic grapevine samples were collected from vineyards in Zanjan province to detect Grapevine virus A. Total RNA was extracted from symptomatic leaf samples and subjected to cDNA synthesis using random hexamer primers. Then, a DNA fragment around 800 bp including the complete coat protein (CP) gene was amplified from nine out of 57 samples by polymerase chain reaction (PCR) using specific ...

full text

Prevalence of Tobacco mosaic virus in Iran and Evolutionary Analyses of the Coat Protein Gene

The incidence and distribution of Tobacco mosaic virus (TMV) and related tobamoviruses was determined using an enzyme-linked immunosorbent assay on 1,926 symptomatic horticultural crops and 107 asymptomatic weed samples collected from 78 highly infected fields in the major horticultural crop-producing areas in 17 provinces throughout Iran. The results were confirmed by host range studies and re...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 11  issue 2

pages  109- 114

publication date 2013-04-01

By following a journal you will be notified via email when a new issue of this journal is published.

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023